Journal: Biomaterials Research

Article Title: Transdermal Delivery of Chinese Medicinal Formula Mitigates Pediatric Constipation by Modulating Intestinal Endocrine and Metabolic Homeostasis

doi: 10.34133/bmr.0374

Figure Lengend Snippet: The areca nut, stir-fried morning glory seeds, rhubarb, and ginseng released from YNJP work synergistically to enhance the secretion of endocrine homeostatic transmitters; regulate mucin, aquaporin, and tight junction protein expression; and alleviate constipation by modulating intestinal metabolic homeostasis effects.

Article Snippet: Rhubarb, stir-fried morning glory seeds, areca nut, ginseng, and cinnabar were purchased from Shanghai Yuanye Biotechnology Co., Ltd. Polyoxyl (40) stearate and cholesterol were bought from Wuhan Rat Laibao Biotechnology Co., Ltd.

Techniques: Expressing

Journal: Materials Today Bio

Article Title: Bioproduction of ∼10 knt single-stranded DNA for constructing large DNA origami structures

doi: 10.1016/j.mtbio.2026.103092

Figure Lengend Snippet: Schematic illustration of the biosynthesis of long ssDNA. The PCR-amplified dsDNA fragments were first assembled into recombinant phagemids using Gibson assembly and subsequently transformed into E. coli for amplification. This process was carried out in both shake-flask cultures and bioreactor systems, with systematic optimization of cultivation conditions to markedly enhance phage particle production. Finally, the resulting ssDNA was harvested and purified, and subsequently employed as a scaffold strand for the assembly of large-scale DNA origami nanostructures.

Article Snippet: Milligram-scale production of synthetic phage particles was carried out in a stirred-tank bioreactor (TMAXTREE Tmax Bio-3L) with a working volume of 1 L. XL1-Blue cells harboring the correctly assembled phagemid were grown to an OD 600 of 0.5, and 4 mL of this culture was inoculated into 2 × YT medium supplemented with 100 μg/mL ampicillin.

Techniques: Amplification, Recombinant, Transformation Assay, Purification

Journal: Materials Today Bio

Article Title: Bioproduction of ∼10 knt single-stranded DNA for constructing large DNA origami structures

doi: 10.1016/j.mtbio.2026.103092

Figure Lengend Snippet: Bioreactor production of 10,563 nt ssDNA. (a) Schematic illustration of ssDNA biosynthesis in a bioreactor system. (b) Growth curve of E. coli during bioreactor cultivation, showing cell density as a function of cultivation time. (c) Effect of bioreactor cultivation time on ssDNA yield, showing a maximum ssDNA production at 30 h of cultivation. All data are presented as mean ± SD (n = 3 biological replicates). Error bars represent standard deviations. (d) Comparison of ssDNA yields obtained using the bioreactor-based approach and reported methods .

Article Snippet: Milligram-scale production of synthetic phage particles was carried out in a stirred-tank bioreactor (TMAXTREE Tmax Bio-3L) with a working volume of 1 L. XL1-Blue cells harboring the correctly assembled phagemid were grown to an OD 600 of 0.5, and 4 mL of this culture was inoculated into 2 × YT medium supplemented with 100 μg/mL ampicillin.

Techniques: Comparison